Karl Haushalter

Associate Professor of Chemistry and Biology

Jacobs Science Center, Room 1218
301 Platt Blvd.
Claremont, CA 91711
909.607.3928
Karl_Haushalter@hmc.edu

Education & professional experience

  • B.A., Rice University
  • Ph.D., Harvard University
  • Visiting Lecturer, University of California, Irvine
  • Postdoctoral Fellow, University of California, San Diego

Teaching

  • Bio 182: Chemistry in Living Systems
  • Bio 184: Methods in Biochemistry
  • Bio 189: Topics in Biochemistry and Molecular Biology
  • Chem 21: General Chemistry
  • Chem 25: General Chemistry Laboratory
  • Chem 58: Carbon Compounds Laboratory
  • Chem 105: Organic Chemistry
  • Chem 111: Organic Chemistry Laboratory

Research Interests

Current approaches to treating HIV-AIDS require patients to take a combination of antiretrovial medicines for the rest of their lives.  If a safe and effective gene therapy strategy could be developed, it would allow patients to better control their disease without a daily and expensive pill burden.  Students in Prof. Karl Haushalter’s lab are helping to develop a lentivirus delivery vehicle that could potentially be used to genetically engineer hematopoietic stem cells (HSC’s) harvested from patients.  After being genetically modified and expanded in culture, the resulting HSC’s could then be reinfused into the patient to reconstitute the patient’s immune system with cells that are resistant to infection by HIV.   This work is being pursued in collaboration with Dr. John Rossi and Dr. Lisa Scherer at the City of Hope National Medical Center.

In addition to these scientific pursuits, Prof. Haushalter is active in community efforts to combat the stigma associated with HIV-AIDS and support those living with HIV-AIDS through education and empowerment.

Recent Student Projects

Matthew Hoss (’08) and Sara Hummel (’06): “Sequence Specificity of Human 8-Oxoguanine DNA Glycosylase”

Katie Mouzakis (’07), Yan Pu (’06), and Tiffany Wu (’06): “DNA Repair Efficiency of Variants of Human 8-oxoguanine DNA Glycosylase 1 (hOGG1) Identified in Cancer Patients”

Fang-Yuan Chang (’07): “Trapping the hOGG1:nucleosomal DNA complex”

Brianna Lyon-Robers (’06): “Improved methods for in vitro nucleosome reconstitution”

Steven Petesch (’05): “Location mechanism of adenine·8-oxoguanine mismatches by MutY adenine DNA glycosylase”

Sarah Bundick (’05) and Ryan Mashiyama (’04): “Target Location by the DNA Repair Enzyme SMUG1”
Kit Rodolfa (’04): “Synthesis of large fragments of DNA incorporating site-specific base lesions”

Alexis Kaushansky (’04): “Filling in the black box between I-CreI induced DNA cleavage and double-strand break repair”

Selected Publications

Gemmen, G. J., R. Sim, K. A. Haushalter, P. C. Ke, J. T. Kadonaga and D. E. Smith. 2005. Forced unraveling of nucleosomes assembled on heterogeneous DNA using core histones, NAP-1, and ACF. J. Mol. Biol. 351: 89-99. [abstract]

Wibley, J. E. A., T. R. Waters, K. Haushalter, G. L. Verdine and L. H. Pearl. 2003. Structure and specificity of the vertebrate anti-mutator uracil-DNA glycosylase SMUG1. Mol. Cell. 11(6): 1647-1659. [article]

K. A. Haushalter and J. T. Kadonaga. 2003. Chromatin assembly by DNA-translocating motors. Nat. Rev. Mol. Cell Biol. 4(8): 613-620. [abstract]

L. Chen, K. A. Haushalter, C. M. Lieber, and G. L. Verdine. 2002. Direct visualization of a DNA glycosylase searching for damage. Chem. Biol. 9: 345-350. [article]

H. Nilsen, K. A. Haushalter, P. Robins, D. E. Barnes, G. L. Verdine and T. Lindahl. 2001. Excision of deaminated cytosine from the vertebrate genome: role of the SMUG1 uracil DNA glycosylase. EMBO J. 20: 4278-4286. [abstract]

K. A. Haushalter, P. T. Stukenberg, M. W. Kirschner and G. L. Verdine. 1999. Identification of a new uracil DNA glycosylase family by expression cloning using synthetic inhibitors. Curr. Biol. 9: 174-185. [article]

K. A. Haushalter, J. Lau, and J. D. Roberts. 1996. An NMR investigation of the effect of hydrogen bonding on the rates of rotation about the C-N bonds in urea and thiourea. J. Amer. Chem. Soc. 118: 8891-8896. [abstract]