Articles

Ogawa, K., D.J. Asai, and C.J. Brokaw. 1977. Properties of an antiserum against native dynein 1 from sea urchin sperm flagella. J. Cell Biol. 73: 182-192.

Asai, D.J. and C.J. Brokaw. 1980. Effects of antibodies against tubulin on the movement of reactivated sea urchin sperm flagella. J. Cell Biol. 87: 114-123.

Wang, C., D.J. Asai, and E. Lazarides. 1980. The 68,000-dalton neurofilament-associated protein is a component of nonneural cells and of skeletal myofibrils. Proc. Nat. Acad. Sci. USA 77: 1541-1545.

O’Connor, C.M., D.L. Gard, D.J. Asai, and E. Lazarides. 1981. Phosphorylation of the intermediate filament proteins, desmin and vimentin, in muscle cells. Cell Prolif. 8: 1157-1169.

O’Connor, C.M., D.J. Asai, C.N. Flytzanis, and E. Lazarides. 1981. Translation of the intermediate filament proteins, desmin and vimentin, in vitro. Mol. Cell Biol. 1: 303-309.

Okuno, M., D.J. Asai, K. Ogawa, and C.J. Brokaw. 1981. Effects of antibodies against dynein and tubulin on the stiffness of flagellar axonemes. J. Cell Biol. 91: 689-694.

Asai, D.J., C.J. Brokaw, R.C. Harmon, and L. Wilson. 1982. Monoclonal antibodies to tubulin and their effects on the movement of reactivated sea urchin spermatozoa. Cell Motility 1: 175-180.

Asai, D.J., C.J. Brokaw, W.C. Thompson, and L. Wilson. 1982. Two different monoclonal antibodies to alpha-tubulin inhibit the bending of reactivated sea urchin spermatozoa. Cell Motility 2: 599-614.

Thompson, W.C., D.J. Asai, and D.H. Carney. 1984. Heterogeneity among microtubules of the cytoplasmic microtubule complex detected by a monoclonal antibody to alpha tubulin. J. Cell Biol. 98: 1017-1025.

Asai, D.J. and L. Wilson. 1985. A latent activity dynein-like cytoplasmic magnesium adenosine triphosphatase. J. Biol. Chem. 260: 699-702.

Asai, D.J., W.C. Thompson, L. Wilson, C.F. Dresden, H. Schulman, and D.L. Purich. 1985. Microtubule-associated proteins (MAPs): A monoclonal antibody to MAP1 decorates microtubules in vitro but stains stress fibers and not microtubules in vivo. Proc. Nat. Acad. Sci. USA 82: 1434-1438.

Asai, D.J., R.J. Leslie, and L. Wilson. 1986. Dynein-like cytoplasmic microtubule translocators. Ann. N.Y. Acad. Sci. 466: 275-291.

Asai, D.J., W.C. Thompson, D.L. Purich, and L. Wilson. 1986. Microtubule-associated proteins: In vitro isolation versus in vivo function. Ann. N.Y. Acad. Sci. 466: 410-412.

O’Brien, E.T., D.J. Asai, A. Groweiss, B.H. Lipshutz, W. Fenical, R.S. Jacobs, and L. Wilson. 1986. Mechanism of action of the marine natural product stypoldione: Evidence for reaction with sulfhydryl groups. J. Med. Chem. 29: 1851-1855.

Ball, R.L., D.H. Carney, T. Albrecht, D.J. Asai, and W.C. Thompson. 1986. A radiolabeled monoclonal antibody binding assay for cytoskeletal tubulin in cultured cells. J. Cell Biol. 103: 1033-1041.

Asai, D.J. 1986. An antiserum to the sea urchin 20S egg dynein reacts with embryonic ciliary dynein but it does not react with the mitotic apparatus. Dev. Biol. 118: 416-424.

Schulze, E., D.J. Asai, J.C. Bulinski, and M. Kirschner. 1987. Post-translational modification and microtubule stability. J. Cell Biol. 105: 2167-2177.

Foltz, K.R. and D.J. Asai. 1988. Ionic strength-dependent isoforms of sea urchin egg dynein. J. Biol. Chem. 263: 2878-2883.

Schatten, G., C. Simerly, D.J. Asai, E. Szoke, P. Cooke, and H. Schatten. 1988. Acetylated alpha-tubulin in microtubules during mouse fertilization and early development. Dev. Biol. 130: 74-86.

Boyce, W.M., D.J. Asai, J.K. Wilder, and K.R. Kazacos. 1989. Physicochemical characterization and monoclonal and polyclonal antibody recognition of Baylisascaris procyonis larval excretory-secretory antigens. J. Parasitol. 75: 540-548.

O’Brien, E.T., D.J. Asai, R.S. Jacobs, and L. Wilson. 1989. Selective inhibition of cytokinesis in sea urchin embryos by low concentrations of stypoldione, a marine natural product that reacts with sulfhydryl groups. Molec. Pharm. 35: 635-642.

Asai, D.J. and N.M. Remolona. 1989. Tubulin isotype usage in vivo: A unique spatial distribution of the minor neuronal-specific beta-tubulin isotype in pheochromocytoma cells. Dev. Biol. 139: 398-409.

Foltz, K.R. and D.J. Asai. 1990. Molecular cloning and expression of sea urchin embryonic ciliary dynein β heavy chain. Cell Motil. Cytoskel. 16: 33-46.

Gibbons, I.R., B.H. Gibbons, G. Mocz, and D.J. Asai. 1991. Multiple nucleotide-binding sites in the sequence of dynein β heavy chain. Nature 352: 640-643.

Gibbons, I.R., D.J. Asai, N.S. Ching, G.J. Dolecki, G. Mocz, C.A. Phillipson, H. Ren, W.-J.Y. Tang, and B.H. Gibbons. 1991. A PCR procedure to determine the sequence of large polypeptides by rapid walking through a cDNA library. Proc. Nat. Acad. Sci. USA 88: 8563-8567.

Beckwith, S.M. and D.J. Asai. 1993. The ciliary dynein of Paramecium tetraurelia: Photolytic maps of the three heavy chains. Cell Motil. Cytoskel. 24: 29-38.

Gibbons, I.R., D.J. Asai, W.-J.Y. Tang, and B.H. Gibbons. 1992. A cytoplasmic dynein heavy chain in sea urchin embryos. Biol. Cell 76: 303-309.

Asai, D.J. and C.J. Brokaw. 1993. Dynein heavy chain isoforms and axonemal motility. Trends in Cell Biology 3: 398-402.

Asai, D.J. and J.K. Wilder. 1993. Making monoclonal antibodies. Methods in Cell Biology 37: 57-74.

Asai, D.J. 1994. Loading the dice. A review of Designing Antibodies by R.D. Mayforth. BioEssays 16: 447-448.

Asai, D.J. 1994. Where in the cell is it? A review of Immunocytochemistry edited by J.E. Beesley. Trends in Cell Biology 4: 268.

Gibbons, B.H., D.J. Asai, W.-J.Y. Tang, T.S. Hays, and I.R. Gibbons. 1994. Phylogeny and expression of axonemal and cytoplasmic dynein genes in sea urchins. Molec. Biol. Cell 5: 57-70.

Keating, H.H. and D.J. Asai. 1994. Distribution of phosphorylated microtubule-associated protein 1B during neurite outgrowth in PC12 cells. Dev. Biol. 162: 143-153.

Asai, D.J., S.M. Beckwith, K.A. Kandl, H.H. Keating, H. Tjandra, and J.D. Forney. 1994. The dynein genes of Paramecium tetraurelia: Sequences adjacent to the catalytic P-loop identify cytoplasmic and axonemal heavy chain isoforms. J. Cell Sci. 107: 839-847.

Asai, D.J. and P.S. Criswell. 1995. Identification of new dynein heavy chain genes by RNA-directed PCR. Methods in Cell Biology 47: 579-585.

Wang, C., D.J. Asai, and K.R. Robinson. 1995. Retrograde but not anterograde bead movement in intact axons requires dynein. J. Neurobiol. 27: 216-226.

Kandl, K.A., J.D. Forney, and D.J. Asai. 1995. The dynein genes of Paramecium tetraurelia: The structure and expression of the ciliary β and cytoplasmic heavy chains. Mol. Biol. Cell 6: 1549-1562.

Asai, D.J. and S.-w. Lee. 1995. The structure and function of dynein heavy chains. Mol. Cells 5: 299-305.

Asai, D.J. Multi-dynein hypothesis. 1995. Cell Motil. Cytoskel. 32: 129-132.

Andrews, K.L., P. Nettesheim, D.J. Asai, and L.E. Ostrowski. 1996. Identification of seven rat axonemal dynein heavy chain genes: Expression during ciliated cell differentiation. Mol. Biol. Cell 7: 71-79.

Peters, J.D., M.T. Furlong, D.J. Asai, M.L. Harrison, and R.L. Geahlen. 1996. Syk, activated by cross-linking the B-cell antigen receptor, localizes to the cytosol where it interacts with and phosphorylates alpha-tubulin on tyrosine. J. Biol. Chem. 271: 4755-4762.

Asai, D.J. 1996. Functional and molecular diversity of dynein heavy chains. Sems. Cell Biol. 7: 311-320.

Karcher, S.J., D.J. Asai, and C.J. Staiger. 1996. Expression vectors used in project-oriented teaching laboratories. In: J.C. Glase, ed., Tested Studies for Laboratory Teaching 17: 1-21.

Criswell, P.S., L.E. Ostrowski, and D.J. Asai. 1996. A novel cytoplasmic dynein heavy chain: Expression of DHC1b in mammalian ciliated epithelial cells. J. Cell Sci. 109: 1891-1898.

Criswell, P.S. and D.J. Asai. 1998. Evidence for four cytoplasmic dynein heavy chain isoforms in rat testis. Mol. Biol. Cell 9: 237-247.

Lee, S.-w., J.C. Wisniewski, W.L. Dentler, and D.J. Asai. 1999. Gene knockouts reveal separate functions for two cytoplasmic dyneins in Tetrahymena thermophila. Mol. Biol. Cell 10: 771-784.

Gibson, T.M. and D.J. Asai. 2000. Isolation and characterization of 22S outer arm dynein from Tetrahymena cilia. Meth. Cell Biol. 62: 433-440.

Sailaja, G., L.M. Lincoln, J. Chen, and D.J. Asai. 2000. Evaluating the dynein heavy chain gene family in Tetrahymena. Meth. Mol. Biol. 161: 17-27.

Asai, D.J., K.M. DeWall, L.M. Lincoln, and R.K. Smith. 2000. Manipulating dynein genes in Tetrahymena. Meth. Mol. Biol. 161: 269-278.

Asai, D.J. 2000. Analysis of dynein structure and function in ciliated protozoa. Japan. J. Protozool. 33: 15-27.

Faruki, S., R.H. Geahlen, and D.J. Asai. 2000. Syk-dependent phosphorylation of microtubules in activated B-lymphocytes. J. Cell Sci. 113: 2557-2565.

Asai, D.J. 2001. Cytoplasmic dynein-2 and ciliogenesis. In: M. Salathe, P. Satir, eds. Cilia, Mucus, and Mucociliary Interactions. M. Dekker, New York. pp. 5-17.

Asai, D.J. and M. Koonce. 2001. The dynein heavy chain: structure, mechanics and evolution. Trends Cell Biol. 11: 196-202.

Ma, H., T.M. Yankee, J. Hu, D.J. Asai, M.L. Harrison, and R.L. Geahlen. 2001. Visualization of Syk-antigen receptor interactions using green fluorescent protein: differential roles for Syk and Lyn in the regulation of receptor capping and internalization. J. Immunol. 166: 1507-1516.

S. Toba, T.M. Gibson, K. Shiroguchi, Y.Y. Toyoshima, and D.J. Asai. 2004. Properties of the full-length heavy chains of Tetrahymena ciliary outer arm dynein separated by urea treatment. Cell Motil. Cytoskel. 58: 30-38.

Asai, D.J. and D.E. Wilkes. 2004. The dynein heavy chain family. J. Euk. Microbiol. 51: 23-29.

Wilkes, D.E. and D.J. Asai. 2004. Dynein organization. Recent Res. Devel. Cell Sci. 1: 141-152.

Karcher, S.J., D.J. Asai, and C.J. Staiger. 2004. Expression vectors used in project-oriented teaching laboratories. In: M.A. O’Donnell, ed., Tested Studies for Laboratory Teaching 25: 41-63.

Adhiambo, C., J.D. Forney, D.J. Asai, and J.H. LeBowitz. 2005. The two cytoplasmic dynein-2 isoforms in Leishmania mexicana perform separate functions. Mol. Biochem. Parasitol. 143: 216-225.

Eisen, J., R. S. Coyne, M. Wu, D. Wu, M. Thiagarajan et al. 2006. Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a model eukaryote. PLoS Biology 4: e286.

Haushalter, K. and D.J. Asai. 2006. Beyond Bio2010: If we build it, will they come? CUR Quarterly 26: 160-163.

Wilkes, D.E., V. Rajagopalan, E. Kniazeva, A. Wiedeman, and D.J. Asai. 2006. The dynein light chain family in Tetrahymena thermophila. Cell Motil. Cytoskel. (in press)

Books edited

Asai, D.J., Editor. 1993. Antibodies in Cell Biology. Methods in Cell Biology, Vol. 37, Academic Press, 452 pages.

Asai, D.J. and J.D. Forney, Editors. 2000. Tetrahymena thermophila. Methods in Cell Biology, Vol. 62, Academic Press, 580 pages.